HITS Solutions - Egypt - Cairo

Experience: 1 to 2 years

Salary: Confidential

Employment: Full Time

Education: Bachelor's Degree","translations":{"name":{"ar":"درجة البكالوريوس","en":"Bachelor's Degree

Design highly specific and uniform primers and multiplex PCR amplicon panels for targeted resequencing of cancer genes, hotspot mutations, fusions, CNVs, and other variants of interest.
Perform in silico validation of primers/probes using tools such as Primer3, NCBI Primer-BLAST, BLAST, UCSC In-Silico PCR, Primer-BLAST, mfold/UNAfold, OligoAnalyzer, AutoDimerv1, Multiple Primer Analyzer, etc.
Assess and minimize primer-dimer formation, off-target amplification, GC bias, and coverage imbalances in multiplex reactions.
Design and maintain custom target BED files, manifest files, and panel metadata compatible with major NGS pipelines.
Collaborate with wet-lab scientists to troubleshoot failed targets and iteratively improve panel performance (coverage, uniformity, sensitivity for low-VAF mutations).
(Optional but valued) Design qPCR/RT-qPCR primers and TaqMan/Molecular Beacon probes for validation or orthogonal confirmation of mutations.
Document all designs, parameters, and performance metrics in a clear, reproducible manner (version-controlled repositories preferred).
Stay up to date with new tools (e.g., Primer Pooler, Pane Lapp, Open Primer, Amplicons, Panel Design, etc.) and publications on primer/design best practices.

Post date: 24 December 2025

Publisher:

Post date: 24 December 2025

Publisher: